Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| transient receptor potential cation channel, subfamily C, member 7 | Polyclonal | IgG | Rabbit | Human, Mouse | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human TRP 7 recombinant protein (Position: K716-I862). Human TRP 7 shares 95% amino acid (aa) sequence identity with mouse TRP 7. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
TRPC7 |
Protein |
Short transient receptor potential channel 7 |
Uniprot ID |
Q9HCX4 |
Function |
Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) (By similarity). May also be activated by intracellular calcium store depletion. |
Tissue Specificity |
|
Sub-cellular localization |
Cell membrane. |
Sequence Similarities |
Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC7 sub-subfamily. |
Aliases |
Capacitative calcium channel TRPC7 antibody|cation channel, subfamily C, member 7 antibody|hTRP7 antibody|KNP3 antibody|Likley ortholog of mouse transient receptor potential cation channel subfamily C member 7 antibody|mTRP7 antibody|Putative capacitative calcium channel antibody|Short transient receptor potential channel 7 antibody|Transient receptor potential cation channel subfamily C member 7 antibody|transient receptor potential-related channel 7, a novel putative Ca2+ channel protein antibody|Transient receptor protein 7 antibody|TRP 7 antibody|TRP-7 antibody|TRP7 protein antibody|TRPC 7 antibody|TRPC7 antibody|Trpc7 transient receptor potential cation channel, subfamily C, member 7 antibody|TRPC7_HUMAN antibody|TRPM2 antibody|Trrp8 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- TRP 7 antibody, ASA-B1929, Western blotting
All lanes: Anti TRP 7 (ASA-B1929) at 0.5ug/ml
WB: Recombinant Human TRP 7 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti TRP 7 (ASA-B1929) at 0.5ug/ml
WB: Recombinant Human TRP 7 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti- TRP 7 antibody, ASA-B1929, Western blotting
All lanes: Anti TRP 7 (ASA-B1929) at 0.5ug/ml
Lane 1: Mouse Brain Tissue Lysate at 50ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 99KD
Observed bind size: 99KD
All lanes: Anti TRP 7 (ASA-B1929) at 0.5ug/ml
Lane 1: Mouse Brain Tissue Lysate at 50ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 99KD
Observed bind size: 99KD
