Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| transient receptor potential cation channel, subfamily C, member 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human TRPC3(836-851aa HSFNSILNQPTRYQQI), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
TRPC3 |
Protein |
Short transient receptor potential channel 3(TrpC3) |
Uniprot ID |
Q13507 |
Function |
Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C, and by inositol 1,4,5- triphosphate receptors (ITPR) with bound IP3. May also be activated by internal calcium store depletion. |
Tissue Specificity |
Expressed predominantly in brain and at much lower levels in ovary, colon, small intestine, lung, prostate, placenta and testis. |
Sub-cellular localization |
Membrane; Multi-pass membrane protein. |
Sequence Similarities |
Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC3 sub-subfamily. |
Aliases |
Htrp 3 antibody|Htrp3 antibody|MGC124333 antibody|Mtrp3 antibody|Receptor activated cation channel TRP3 antibody|Short transient receptor potential channel 3 antibody|Transient receptor potential cation channel subfamily C member 3 antibody|Transient receptor potential channel 3 antibody|TRANSIENT RECEPTOR POTENTIAL DROSOPHILA HOMOLOG OF 3 antibody|Transient receptor protein 3 antibody|Trp related protein 3 antibody|TRP3 antibody|TrpC3 antibody|Trrp3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TRPC3 antibody, ASA-B1930, Western blotting
All lanes: Anti TRPC3 (ASA-B1930) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: Rat Intestine Tissue Lysate at 50ug
Lane 4: Rat Ovary Tissue Lysate at 50ug
Lane 5: U87 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: SW620 Whole Cell Lysate at 40ug
Lane 9: HELA Whole Cell Lysate at 40ug
P
All lanes: Anti TRPC3 (ASA-B1930) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Lung Tissue Lysate at 50ug
Lane 3: Rat Intestine Tissue Lysate at 50ug
Lane 4: Rat Ovary Tissue Lysate at 50ug
Lane 5: U87 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: COLO320 Whole Cell Lysate at 40ug
Lane 8: SW620 Whole Cell Lysate at 40ug
Lane 9: HELA Whole Cell Lysate at 40ug
P
Anti-TRPC3 antibody, ASA-B1930, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
