Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| uncoupling protein 1(mitochondrial, proton carrier) | Polyclonal | IgG | Rabbit | Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse UCP1(289-307aa FEQLKKELMKSRQTVDCTT), identical to the related rat sequence. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
UCP1 |
Protein |
Mitochondrial brown fat uncoupling protein 1 |
Uniprot ID |
P04633 |
Function |
UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat. |
Tissue Specificity |
Brown adipose tissue. |
Sub-cellular localization |
Mitochondrion inner membrane; Multi-pass membrane protein. |
Sequence Similarities |
Belongs to the mitochondrial carrier (TC 2.A.29) family. |
Aliases |
mitochondrial brown fat uncoupling protein antibody|Mitochondrial brown fat uncoupling protein 1 antibody|SLC25A7 antibody|Solute carrier family 25 member 7 antibody|Thermogenin antibody|UCP 1 antibody|UCP antibody|UCP1 antibody|UCP1_HUMAN antibody|uncoupling protein 1(mitochondrial, proton carrier) antibody|Uncoupling protein 1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-UCP1 antibody, ASA-B1950, Western blotting
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: Mouse Spleen Tissue Lysate
Lane 4: Mouse Lung Tissue Lysate
Lane 5: HEPA Cell Lysate
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: Mouse Spleen Tissue Lysate
Lane 4: Mouse Lung Tissue Lysate
Lane 5: HEPA Cell Lysate
