VDAC/Porin Antibody

VDAC/Porin Antibody

$330.00

In stock

SKU: ASA-B1958 Category:

Description

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
voltage-dependent anion channel 1 Polyclonal IgG Rabbit Human, Mouse, Rat IHC-P, IHC-F, ICC, WB Immunogen affinity purified.

Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human VDAC(163-178aa RVTQSNFAVGYKTDEF), identical to the related rat and mouse sequences.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 VDAC1

Protein

 Voltage-dependent anion-selective channel protein 1(VDAC-1/hVDAC1)

Uniprot ID

 P21796

Function

 Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.

Tissue Specificity

Heart, liver and skeletal muscle.

Sub-cellular localization

 Mitochondrion outer membrane.

Sequence Similarities

 Belongs to the eukaryotic mitochondrial porin family.

Aliases

 N2441 antibody|OMP2 antibody|POR1 antibody|hVDAC1 antibody|MGC111064 antibody|Mitochondrial Porin antibody|Outer mitochondrial membrane protein porin 1 antibody|Plasmalemmal porin antibody|Porin 31 HL antibody|Porin 31HL antibody|Porin 31HM antibody|VDAC 1 antibody|VDAC antibody|VDAC-1 antibody|VDAC1 antibody|VDAC1_HUMAN antibody|Voltage dependent anion channel 1 antibody|Voltage dependent anion selective channel protein 1 antibody|Voltage-dependent anion-selective channel protein 1 antibody|YNL055C antibody|YNL2441C antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Mouse, Rat AssaySolutio’s ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, Mouse, Rat AssaySolutio’s IHC/ICC Detection kit
Immunohistochemistry(Frozen Section) 0.5-1μg/ml Rat Human, Mouse AssaySolutio’s IHC/ICC Detection kit
Immunocytochemistry 0.5-1μg/ml Human, Mouse Rat AssaySolutio’s IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information

Anti-VDAC/Porin antibody, ASA-B1958, Western blotting
All lanes: Anti VDAC/Porin (ASA-B1958) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Rat Heart Tissue Lysate at 50ug
Lane 3: Rat Liver Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: A431 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: SMMC Whole Cell Lysate at 40ug
Lane 8: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 31KD<br
Anti-VDAC/Porin antibody, ASA-B1958, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
Anti-VDAC/Porin antibody, ASA-B1958, IHC(P)
IHC(P): Rat Skeletal Muscle Tissue

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