Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| VGF nerve growth factor inducible | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human VGF(582-600aa QARRAQEEAEAEERRLQEQ), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
VGF |
Protein |
Neurosecretory protein VGF |
Uniprot ID |
O15240 |
Function |
May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system. |
Tissue Specificity |
Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells. |
Sub-cellular localization |
Secreted. |
Sequence Similarities |
|
Aliases |
NERP-1 antibody|NERP-2 antibody|Nerve growth factor inducible antibody|Neuroendocrine regulatory peptide-2 antibody|Neurosecretory protein VGF antibody|VGF antibody|VGF_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-VGF antibody, ASA-B1972, Western blotting
Lane 1: Rat Brain Tissue
Lane 2: U87 Cell Lysate
Lane 3: U87 Cell Lysate
Lane 4: SHG Cell Lysate
Lane 5: NEURO Cell Lysate
Lane 1: Rat Brain Tissue
Lane 2: U87 Cell Lysate
Lane 3: U87 Cell Lysate
Lane 4: SHG Cell Lysate
Lane 5: NEURO Cell Lysate
