Vinculin Antibody (monoclonal)

Vinculin Antibody (monoclonal)

$330.00

In stock

SKU: ASA-B1976 Category:

Description

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
vinculin Monoclonal IgG Mouse Chicken, Human, Mouse, Rat IHC-F, WB Ascites

Immunogen
Human vinculin, purified from uterus.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.

Concentration

 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 VCL

Protein

 Vinculin

Uniprot ID

 Human(P18206), Mouse(Q64727), Rat(P85972)

Function

 Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

Tissue Specificity

Metavinculin is muscle-specific.

Sub-cellular localization

 Cytoplasm, cytoskeleton.

Sequence Similarities

 Belongs to the vinculin/alpha-catenin family.

Aliases

 MVCL|CMD1W|CMH15|metavinculin

Application Details

Application Concentration* Species Validated Using**
Western blot 1-2μg/ml Human, chicken, mouse, rat AssaySolutio’s ECL kit
Immunohistochemistry(Frozen Section) 2-4μg/ml Human, chicken, mouse, rat AssaySolutio’s IHC/ICC Detection kit

AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information

Anti- Vinculin antibody, ASA-B1976, IHC(P)
IHC(P): Human Placenta Tissue
Anti- Vinculin antibody, ASA-B1976, IHC(P)
IHC(P): Human Placenta Tissue

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