Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| vasoactive intestinal peptide receptor 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human VIP Receptor 1(400-414aa RRKWRRWHLQGVLGW), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
VIPR1 |
Protein |
Vasoactive intestinal polypeptide receptor 1(VIP-R-1) |
Uniprot ID |
P32241 |
Function |
This is a receptor for VIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The affinity is VIP : PACAP-27 > PACAP-38. |
Tissue Specificity |
In lung, HT-29 colonic epithelial cells, Raji B-lymphoblasts. Lesser extent in brain, heart, kidney, liver and placenta. Not expressed in CD4+ or CD8+ T-cells. Expressed in the T-cell lines HARRIS, HuT 78, Jurkat and SUP-T1, but not in the T- cell lines Peer, MOLT-4, HSB and YT. |
Sub-cellular localization |
Cell membrane; Multi-pass membrane protein. |
Sequence Similarities |
|
Aliases |
FLJ41949 antibody|HVR 1 antibody|HVR1 antibody|PACAP R 2 antibody|PACAP type II receptor antibody|PACAP-R-2 antibody|PACAP-R2 antibody|Pituitary adenylate cyclase activating polypeptide receptor type II antibody|Pituitary adenylate cyclase activating polypeptide type II receptor antibody|Pituitary adenylate cyclase-activating polypeptide type II receptor antibody|RCD1 antibody|RDC 1 antibody|RDC1 antibody|V1RG antibody|VAPC 1 antibody|VAPC1 antibody|Vasoactive intestinal peptide receptor 1 antibody|Vasoactive intestinal polypeptide receptor 1 antibody|Vasoactive intestinal polypeptide receptor 1 precursor antibody|VIP R 1 antibody|VIP receptor type I antibody|VIP-R-1 antibody|VIPR 1 antibody|VIPR antibody|VIPR1 antibody|VIPR1_HUMAN antibody|VIRG antibody|VPAC 1 antibody|VPAC1 antibody|VPCAP1R antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- VIPR1 antibody, ASA-B1978, Western blotting
All lanes: Anti VIPR1 (ASA-B1978) at 0.5ug/ml
WB: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 52KD
Observed bind size: 52KD
All lanes: Anti VIPR1 (ASA-B1978) at 0.5ug/ml
WB: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 52KD
Observed bind size: 52KD
