Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| wingless-type MMTV integration site family, member 3A | Polyclonal | IgG | Rabbit | Human, Mouse | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Wnt3a(252-267aa WVETLRPRYTYFKVPT), identical to the related mouse sequence. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
WNT3A |
Protein |
Protein Wnt-3a |
Uniprot ID |
P56704 |
Function |
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. |
Tissue Specificity |
Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. |
Sub-cellular localization |
Secreted, extracellular space, extracellular matrix. |
Sequence Similarities |
Belongs to the Wnt family. |
Aliases |
MGC119418 antibody|MGC119419 antibody|MGC119420 antibody|Protein Wnt 3a Precursor antibody|Protein Wnt-3a antibody|Wingless type MMTV integration site family member 3A antibody|Wnt 3a antibody|wnt3a antibody|WNT3A protein antibody|WNT3A_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-WNT3A antibody, ASA-B1992, Western blotting
All lanes: Anti WNT3A (ASA-B1992) at 0.5ug/ml
WB: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti WNT3A (ASA-B1992) at 0.5ug/ml
WB: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 39KD
Observed bind size: 39KD
