Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| Yip1 domain family, member 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human YIPF3(47-61aa FEDMGELHQRLREEE), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
YIPF3 |
Protein |
Protein YIPF3 |
Uniprot ID |
Q9GZM5 |
Function |
Involved in the maintenance of the Golgi structure. May play a role in hematopoiesis. |
Tissue Specificity |
Expressed by nucleated hematopoietic cells (at protein level). |
Sub-cellular localization |
Cell membrane; Multi-pass membrane protein. Cytoplasm. Golgi apparatus, cis-Golgi network membrane; Multi-pass membrane protein. Note: Localization to the cytoplasm or to the cell membrane is developmentally and ontogenetically regulated. |
Sequence Similarities |
Belongs to the YIP1 family. |
Aliases |
Killer lineage protein 1 antibody|Natural killer cell-specific antigen KLIP1 antibody|Protein YIPF3 antibody|YIP1 family member 3 antibody|Yipf3 antibody|YIPF3_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-YIPF3 antibody, ASA-B2005, Western blotting
All lanes: Anti YIPF3 (ASA-B2005) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Thymus Tissue Lysate at 50ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 48KD
Observed bind size: 35KD
All lanes: Anti YIPF3 (ASA-B2005) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Thymus Tissue Lysate at 50ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 48KD
Observed bind size: 35KD
Anti-YIPF3 antibody, ASA-B2005, IHC(P)
IHC(P): Rat Testis Tissue
IHC(P): Rat Testis Tissue
