Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| zinc finger E-box binding homeobox 2 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human ZEB2 recombinant protein (Position: M1-P200). Human ZEB2 shares 92% amino acid (aa) sequence identity with mouse ZEB2. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
ZEB2 |
Protein |
Zinc finger E-box-binding homeobox 2 |
Uniprot ID |
O60315 |
Function |
Transcriptional inhibitor that binds to DNA sequence 5′- CACCT-3′ in different promoters. Represses transcription of E- cadherin. |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family. |
Aliases |
FLJ42816 antibody|HSPC082 antibody|KIAA0569 antibody|SIP 1 antibody|SIP1 antibody|Smad-interacting protein 1 antibody|SMADIP 1 antibody|SMADIP1 antibody|ZEB 2 antibody|ZEB2 antibody|ZEB2_HUMAN antibody|Zfhx1b antibody|ZFHX1B protein antibody|Zfx1b antibody|Zinc finger E box binding protein 2 antibody|Zinc finger E-box-binding homeobox 2 antibody|Zinc finger homeobox 1b antibody|zinc finger homeobox protein 1 antibody|Zinc finger homeobox protein 1b antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- ZEB2antibody, ASA-B2006, Western blotting
All lanes: Anti ZEB2 (ASA-B2006) at 0.5ug/ml
WB: Recombinant Human ZEB2 Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
All lanes: Anti ZEB2 (ASA-B2006) at 0.5ug/ml
WB: Recombinant Human ZEB2 Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
Anti- ZEB2antibody, ASA-B2006, Western blotting
All lanes: Anti ZEB2 (ASA-B2006) at 0.5ug/ml
WB: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 136KD
Observed bind size: 136KD
All lanes: Anti ZEB2 (ASA-B2006) at 0.5ug/ml
WB: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 136KD
Observed bind size: 136KD
